Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription

Abstract

FUS/EWS/TAF15 (FET) fusion oncoproteins contain low complexity domain which forms biomolecular condensates that recruit RNA polymerase II. Here the authors develop a single-molecule assay to visualize this phenomenon providing in vitro evidence to support causative relationship between the formation of condensates on DNA and gene transcription. Furthermore, they also determine a threshold number of fusion-binding DNA satellite elements required for the formation of FET protein condensates.