Jichu yixue yu linchuang (May 2020)

Preliminary study on the mechanism of RNA-binding protein as a drug target of clofoctol

  • ZHANG Mei-lian, WANG Zhi-xing, HU Yan, RAN Kun-nian, HAN Wei, PENG Xiao-zhong

Journal volume & issue
Vol. 40, no. 5
pp. 621 – 626

Abstract

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Objective To explore the molecular mechanism of anti-glioma by clofoctol targeting on RNA-binding proteins. Methods Biotin pull-down experiments were used to detect the binding of RNA-binding protein UNR (upstream of N-ras) to the target gene Krüppel-like factor 13(KLF13) mRNA fragments, and the effect of clofoctol on the binding to UNR to KLF13 mRNA fragments; The results of clofoctol DARTS sample's mass spectrometry combined with transcriptome chip analysis were used to screen other RNA-binding protein targets and to predict other downstream target genes. Results Clofoctol enhanced the interaction between UNR and the fragment of KLF13 mRNA; Clofoctol also acted on other RNA-binding proteins, and functioned in mTOR signaling pathway and TNF-related apoptosis-inducing ligand (TRAIL)-activated apoptotic signaling pathway. Conclusions Clofoctol can resist gliomas by acting on RNA-binding proteins.

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