Feedforward growth rate control mitigates gene activation burden

Carlos Barajas; Hsin-Ho Huang; Jesse Gibson; Luis Sandoval; Domitilla Del Vecchio

doi:10.1038/s41467-022-34647-1

Nature Communications (Nov 2022)

Feedforward growth rate control mitigates gene activation burden

Carlos Barajas,
Hsin-Ho Huang,
Jesse Gibson,
Luis Sandoval,
Domitilla Del Vecchio

Affiliations

Carlos Barajas: Department of Mechanical Engineering, Massachusetts Institute of Technology
Hsin-Ho Huang: Department of Mechanical Engineering, Massachusetts Institute of Technology
Jesse Gibson: Department of Bioengineering, Stanford University
Luis Sandoval: Department of Chemical Engineering, Massachusetts Institute of Technology
Domitilla Del Vecchio: Department of Mechanical Engineering, Massachusetts Institute of Technology

DOI: https://doi.org/10.1038/s41467-022-34647-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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Heterologous gene activation causes non-physiological burden on cellular resources that cells are unable to adjust to. Here the authors present a tunable, modular, and portable feedforward controller that allows dynamic modulation of a genes expression to possibly high-levels without substantially affecting growth rate.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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