Zhejiang Daxue xuebao. Lixue ban (Nov 2010)

Uniform design for in vitro culture and Plant regeneration through Petals of Fragaria orientalis Lozinsk var. concolor (Kitag.) Liou et C. Y. Li(基于均匀设计优化深山草莓花瓣离体培养及植株再生体系)

  • GUDi-zhou(顾地周),
  • GAOHan-dong(高捍东),
  • ZHUJun-yi(朱俊义),
  • JIANGYun-tian(姜云天),
  • FENGYing(冯颖),
  • SUNZhong-lin(孙忠林),
  • LIUXue-lian(刘雪莲)

DOI
https://doi.org/10.3785/j.issn.1008-9497.2010.06.019
Journal volume & issue
Vol. 37, no. 6
pp. 700 – 704

Abstract

Read online

应用均匀设计法对深山草莓花瓣诱导愈伤组织和愈伤组织再分化芽苗的主要因子和水平进行了探讨.结果表明,深山草莓花瓣愈伤组织诱导的适宜培养基为LS+TDZ 2.06 mg • L-1+2, 4-D 0.60 mg • L-1,诱导率为98%;愈伤组织再分化芽苗的适宜培养基为1/2 LS + TDZ 2.30 mg • L-1 + NAA 0.10 mg • L-1 + KT 1.30 mg • L-1,分化率为96.3%.炼苗移栽后观察植株的形态、花、果及生长等特征,以筛选具有优良性状的株系.

Keywords