Surgical technique for developing a rabbit model of congenital diaphragmatic hernia and tracheal occlusion

M. Mudri; S.A. Smith; C. Vander Tuin; J. Davidson; T.R.H. Regnault; A. Bütter

MethodsX (Jan 2019)

Surgical technique for developing a rabbit model of congenital diaphragmatic hernia and tracheal occlusion

M. Mudri,
S.A. Smith,
C. Vander Tuin,
J. Davidson,
T.R.H. Regnault,
A. Bütter

Affiliations

M. Mudri: Division of General Surgery, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada
S.A. Smith: Division of General Surgery, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada
C. Vander Tuin: Departments of Obstetrics & Gynaecology and Physiology & Pharmacology, Schulich School of Medicine, Western University, London, ON, Canada
J. Davidson: Division of Paediatric General Surgery, Children’s Hospital London Health Science Centre, Schulich School of Medicine, Western University, London, ON, Canada
T.R.H. Regnault: Departments of Obstetrics & Gynaecology and Physiology & Pharmacology, Schulich School of Medicine, Western University, London, ON, Canada
A. Bütter: Division of Paediatric General Surgery, Children’s Hospital London Health Science Centre, Schulich School of Medicine, Western University, London, ON, Canada; Corresponding author at: Western University, Children’s Hospital, London Health Sciences Centre, Rm B1-188, 800 Commissioners Road East, London, ON, N6C 2V3, Canada.

Journal volume & issue: Vol. 6
pp. 594 – 600

Abstract

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The surgical model of congenital diaphragmatic hernia (CDH) has been utilized in exploring treatments and innovative therapies, such as tracheal occlusion (TO). The rabbit is an excellent surgical model compared to others due to lower cost, ease of care, short gestational period, and large litter size. This model is also ideal in studying lung hypoplasia of CDH because rabbit lung development is most similar to humans as alveolarization begins prior to birth and continues post-natally. However, the surgical technique in creating a rabbit model of CDH is quite difficult and information is lacking on how to establish this model. Therefore, the aim of this paper is to describe: • Surgical technique in establishing a rabbit model of CDH and TO • Perioperative care for pregnant rabbit does Method name: Rabbit model of congenital diaphragmatic hernia and tracheal occlusion, Keywords: Congenital diaphragmatic hernia (CDH), Tracheal occlusion (TO), Animal model, Rabbit, Fetal surgery

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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