The influence of chemical polyamines analogs, decarboxylated ornithine and s-(adenosyl)-methionine on the polyamine synthsis velocity in test-systems from tissues with high proliferation

Abstract

The aliphatic (amino)- and (oxy)-derivatives of polyamines effect on the rate of polyamines (Putrescine, Spermidine and Spermine) synthesis in cell-free test-systems from rat's regenerating liver and Hepatoma tissues was examined. The common known inhibitors of polyamines synthesis were also used for the analysis. The research data confirm that cell-free model systems function adequately to applied tests. The effectiveness of anti-proliferate action of chemical compounds was valued by the inhibition degree of Putrescine and polyamines synthesis. In addition, the coefficient of ratio Spermidine (moles)/ Spermine (moles) and the value of total Polyamines pool after 1 h of test-systems incubation with chemical compound were calculated. From the tested compounds 1-aminooxy-3-aminopropan (I), S-(5'-desoxyadenosile)-S-methyl-β-thioethyl-hydroxylamine (IV) and ethylenediamine (V) did satisfied the requirements to a considerable degree.

Keywords

• полиамины
• путресцин
• спермидин
• спермин
• окислительное дезаминирование
• дифторметилорнитин
• орнитиндекарбоксилаза