The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica

Tracy L. Nicholson; Ursula Waack; Ursula Waack; Damarius S. Fleming; Qing Chen; Laura C. Miller; Tod J. Merkel; Scott Stibitz

doi:10.3389/fmicb.2024.1305097

Frontiers in Microbiology (Mar 2024)

The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica

Tracy L. Nicholson,
Ursula Waack,
Ursula Waack,
Damarius S. Fleming,
Qing Chen,
Laura C. Miller,
Tod J. Merkel,
Scott Stibitz

Affiliations

Tracy L. Nicholson: Agricultural Research Service, USDA, National Animal Disease Center, Ames, IA, United States
Ursula Waack: Agricultural Research Service, USDA, National Animal Disease Center, Ames, IA, United States
Ursula Waack: United States Department of Energy, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States
Damarius S. Fleming: USDA, Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD, United States
Qing Chen: Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, United States
Laura C. Miller: Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States
Tod J. Merkel: Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, United States
Scott Stibitz: Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, United States

DOI: https://doi.org/10.3389/fmicb.2024.1305097
Journal volume & issue: Vol. 15

Abstract

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Bordetella bronchiseptica is a highly contagious respiratory bacterial veterinary pathogen. In this study the contribution of the transcriptional regulators BvgR, RisA, RisS, and the phosphorylation of RisA to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation were evaluated. Next Generation Sequencing (RNASeq) was used to differentiate the global gene regulation of both virulence-activated and virulence-repressed genes by each of these factors. The BvgAS system, along with BvgR, RisA, and the phosphorylation of RisA served in cyclic-di-GMP degradation. BvgR and unphosphorylated RisA were found to temporally regulate motility. Additionally, BvgR, RisA, and RisS were found to be required for biofilm formation.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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