Anti-bacterial/fungal and anti-cancer performance of green synthesized Ag nanoparticles using summer savory extract

Abstract

Nowadays, the expansion of nanoparticles as antimicrobial and anticancer agents is one of the most exciting methods for cancer therapy and microbial diseases that can vanquish the problems of drug resistance. In this research, the biosynthesis of silver nanoparticles (AgNPs) was stated using Summer Savory extract. Moreover, these AgNPs by UV-Vis spectroscopy, FTIR, XRD, FESEM, EDX, PSA, and zeta potential analysis were characterized, and by antimicrobial and anti-cancerous performance was evaluated. The maximum absorption peak at 472 nm by UV-Visible spectra confirmed the formation of Ag nanoparticles. The results obtained by FT-IR determined the presence of organic compounds such as amines, alkenes, alcohols, phenols, which performed significant roles in the fixating of these nanoparticles. The results obtained from FESEM, EDX, PSA, and Zeta Potential Analysis revealed that synthesized AgNPs had a hexagonal structure with a size ranging from 10 to 100 nm. The antimicrobial properties of the synthesized AgNPs were examined by Minimum Inhibitory Concentration, Minimum Bactericidal Concentration, and Minimum Fungal Concentration against five microbial strains included Klebsiella pneumonia, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Saccharomyces cerevisiae. The results demonstrated that biosynthesized AgNPs had the most antimicrobial activity, especially against E. Coli. The anti-cancerous results of these nanoparticles through MTT assay demonstrated exceptional anticancer effects against K-562 and MCF-7 human cancer cell lines with IC50 values 50 and 200 µg/ml, respectively.

Keywords

• summer savory
• ag nanoparticle
• green synthesis
• anti-cancer
• anti-bacterial/fungal