Optimization of Simple DNA Extraction Method Suitable for Diverse Microorganisms

Abstract

To date no simple DNA extraction method was reported to be efficient and adapted to various organisms and microorganisms. Moreover, this approach is hard, time consuming and rely on the use of liquid nitrogen. In order to obtain highly purified nucleic acids free of contaminants that could interfere with the amplification reaction during PCR, adequate and easy extraction methods should be developed. During this work, an efficient, fast and economical method for the isolation of high-quality DNA from fungi, bacteria and viruses is described. Those DNA extractions were performed without the use of liquid nitrogen. Besides, the protocol used allowed obtaining very good DNA concentrations that can be utilized at 1/50, regardless the origin of the analyzed samples, whether freshly collected, conserved in calcium chloride, or frozen at -80°C for long time (more than 10 years). The quantity and the quality of the extracted DNA by this method are enough high to perform cloning, PCR simplex or multiplex and also other DNA manipulation techniques.

Keywords

• DNA
• extraction
• liquid nitrogen
• microorganisms
• PCR