PLoS ONE (Jan 2014)

Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?

  • Marta Pokrywczynska,
  • Arkadiusz Jundzill,
  • Jan Adamowicz,
  • Tomasz Kowalczyk,
  • Karolina Warda,
  • Marta Rasmus,
  • Lukasz Buchholz,
  • Sandra Krzyzanowska,
  • Pawel Nakielski,
  • Pawel Nakielski,
  • Tomasz Chmielewski,
  • Magdalena Bodnar,
  • Andrzej Marszalek,
  • Robert Debski,
  • Malgorzata Frontczak-Baniewicz,
  • Grzegorz Mikułowski,
  • Maciej Nowacki,
  • Tomasz A Kowalewski,
  • Tomasz Drewa

DOI
https://doi.org/10.1371/journal.pone.0105295
Journal volume & issue
Vol. 9, no. 8
p. e105295

Abstract

Read online

The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.