Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale

Yuhong Lü; Changwu Yue; Meiyun Shao; Shengyan Qian; Ning Liu; Yuxin Bao; Miao Wang; Minghao Liu; Xiaoqian Li; Yinyin Wang; Ying Huang

doi:10.3390/molecules21060711

Molecules (May 2016)

Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale

Yuhong Lü,
Changwu Yue,
Meiyun Shao,
Shengyan Qian,
Ning Liu,
Yuxin Bao,
Miao Wang,
Minghao Liu,
Xiaoqian Li,
Yinyin Wang,
Ying Huang

Affiliations

Yuhong Lü: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Changwu Yue: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Meiyun Shao: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Shengyan Qian: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Ning Liu: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Yuxin Bao: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Miao Wang: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Minghao Liu: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Xiaoqian Li: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Yinyin Wang: Guizhou Key Laboratory of Microbial Resources & Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China
Ying Huang: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

DOI: https://doi.org/10.3390/molecules21060711
Journal volume & issue: Vol. 21, no. 6
p. 711

Abstract

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Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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