Identification of CELF1 RNA targets by CLIP-seq in human HeLa cells

Abstract

The specific interactions between RNA-binding proteins and their target RNAs are an essential level to control gene expression. By combining ultra-violet cross-linking and immunoprecipitation (CLIP) and massive SoliD sequencing we identified the RNAs bound by the RNA-binding protein CELF1, in human HeLa cells. The CELF1 binding sites deduced from the sequence data allow characterizing specific features of CELF1-RNA association. We present therefore the first map of CELF1 binding sites in human cells.

Keywords

• CUGBP1
• RNA binding proteins
• CLIP-seq
• Homo sapiens
• Alternative splicing