Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production

Yanfei Zhang; Jeremy D. Cortez; Sarah K. Hammer; César Carrasco-López; Sergio Á. García Echauri; Jessica B. Wiggins; Wei Wang; José L. Avalos

doi:10.1038/s41467-021-27852-x

Nature Communications (Jan 2022)

Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production

Yanfei Zhang,
Jeremy D. Cortez,
Sarah K. Hammer,
César Carrasco-López,
Sergio Á. García Echauri,
Jessica B. Wiggins,
Wei Wang,
José L. Avalos

Affiliations

Yanfei Zhang: Department of Chemical and Biological Engineering, Princeton University
Jeremy D. Cortez: Department of Molecular Biology, Princeton University
Sarah K. Hammer: Department of Chemical and Biological Engineering, Princeton University
César Carrasco-López: Department of Chemical and Biological Engineering, Princeton University
Sergio Á. García Echauri: Department of Chemical and Biological Engineering, Princeton University
Jessica B. Wiggins: Genomics Core Facility, Lewis-Sigler Institute for Integrative Genomics, Princeton University
Wei Wang: Genomics Core Facility, Lewis-Sigler Institute for Integrative Genomics, Princeton University
José L. Avalos: Department of Chemical and Biological Engineering, Princeton University

DOI: https://doi.org/10.1038/s41467-021-27852-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

Read online

There are a lack of eukaryotic biosensors specific for branched-chain amino acid (BCAA)-derived products. Here the authors report a genetically encoded biosensor for BCAA metabolism based on the Leu3p transcriptional regulator; they use this to monitor yeast production of isobutanol and isopentanol.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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