Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography

Vaibhao Janbandhu; Ella M.M.A. Martin; Gavin Chapman; Sally L. Dunwoodie; Richard P. Harvey

STAR Protocols (Mar 2022)

Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography

Vaibhao Janbandhu,
Ella M.M.A. Martin,
Gavin Chapman,
Sally L. Dunwoodie,
Richard P. Harvey

Affiliations

Vaibhao Janbandhu: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia; St. Vincent’s Clinical School, UNSW Sydney, Sydney, NSW, Australia; Corresponding author
Ella M.M.A. Martin: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia
Gavin Chapman: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia; St. Vincent’s Clinical School, UNSW Sydney, Sydney, NSW, Australia
Sally L. Dunwoodie: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia; St. Vincent’s Clinical School, UNSW Sydney, Sydney, NSW, Australia; School of Biotechnology and Biomolecular Science, UNSW Sydney, Sydney, NSW, Australia
Richard P. Harvey: Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia; St. Vincent’s Clinical School, UNSW Sydney, Sydney, NSW, Australia; School of Biotechnology and Biomolecular Science, UNSW Sydney, Sydney, NSW, Australia; Corresponding author

Journal volume & issue: Vol. 3, no. 1
p. 101055

Abstract

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Summary: Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dynamics. Here, we present an X-ray microcomputed tomography (micro-CT) workflow that generates accurate volumetric quantification of scar and surviving myocardium in infarcted mouse hearts. This workflow could be applied to other fibrotic organs or hearts from other species.For complete details on the use and execution of this protocol, please refer to Janbandhu et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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