Genetics and amino acid composition in woton plants (Sterculia sp.) from Raja Ampat: an alternative nutrition material for fishes

Abstract

This study aimed to determined the genetic, phylogenetic, and amino acid composition of the woton plant (Sterculia sp) from Raja Ampat that could be utilized in improving nutrition, growth, and fish health. The research used bioinformatics analysis through DNA amplification of the samples of woton plant (WT01 and WT02) with PCR, homology analysis with BLASTN, phylogenetic with Clustal O (1.2.4) program, and analysis of amino acid composition with Expasy Translate Tool, Proparam, Proscale, and compound bioactive with Prosite. The results showed that WT01 (849 bp) 97% identical and WT02 (875 bp) 99% identical were most homologous alignments with Sterculia tragacantha (ID: AY321178.1). WT01 and WT02 had have a 94.21% identity level and were in one group with Sterculia lanceifolia (ID: KR531475.1), Sterculia lanceifolia (ID: KR531477.1) and Sterculia brevissima (ID: KR531473.1). The results used ProtParam that the major amino acid composition in WT01 were 13.9% serine, 12.2% leucine,10% lysine, 7.2% arginine, and 7.2% isoleucine and in WT02 were 12.8% serine, 12.3% leucine, 9.5% lysine, 7.3% arginine, and 7.8% isoleucine. Bioinformatics analysis with Prosite showed that WT01 and WT02 have a small potential of active compound BIG 1(bacterial Ig-like domain 1).

Keywords

• Sterculia
• woton
• genetic
• amino acids
• bioinformatics