CONTENT OF ACTIVE COMPOUNDS, TOXICITY OF CRUDE EXTRACTS OF MANGROVE LEAVES (RHIZOPHORA MUCRONATA) AND ITS MODULATION OF TILAPIA (OREOCHROMIS NILOTICUS)

Abstract

Limited cultivation unit land makes the increase of aquaculture from a widely developed into an intensive system. Intensive system with high density care in confined spaces has the potential to cause stress and disease in fish. Natural ingredients have good criteria such as environmentally friendly, cost-effective, and broad spectrum activities. Alternative natural ingredients in this study using coarse extract Rhizophora mucronata. This study aims to determine the content of active compounds, toxicity values (LC50) and at what doses in such compounds can maximally modulate the immune response (leukocytes) in indigo (Oreochromis niloticus). In this study the extraction method used maceration with methanol solvent p.a. Analysis of biological components is performed by phytochemical test method, UV-Vis spectrophotometer and FT-IR. Phytochemical test results contain two compounds namely flavonoids (+) and alkaloids (+). UV-Vis tests showed the basic structure of flavonoid group compounds (tape I 282 nm, 236 nm and tape II 404 nm) and alkaloids (susceptible to 203 nm-230 nm) by strengthening the emergence of group absorption of functions typical of both compounds using FT-IR. LC50 indicates a dose of 66,364 mg.kg-1. The most significant increase in total leukocytes in test fish (Oreochromis niloticus) was at a dose of 30 mg.kg-1 (2.6 x 104 cells/mm³) with an influence of 91%.

Keywords

• rhizophora mucronata
• flavonoids
• alkaloids
• lc50
• total leucocytes
• oreochromis niloticus