Titer IgG Pertusis pada Usia Remaja, Dewasa, dan Orang Tua Mempergunakan Metode ELISA dan Mikroaglutinasi Pertusis

Abstract

Pertussis antigen which only given in primary immunization, could not protect for a long period. In some countries whooping cough became an reemerging disease. The objective of this study was to evaluate the correlation between age and percentage of positive IgG pertussis in adolescent, adult, and elderly population using enzyme link immunosorbent assay (ELISA) and microagglutination. This observational analytic with cross sectional study was done on 402 subjects, consisted of 134 adolescents (12–18 years), 187 adults (19–49 years), and 81 elderly (50–64 years) in Bandung, period 2008–2010. Titer of pertussis IgG was measured using ELISA and microagglutination of pertussis (MAP). The ELISA results showed positive IgG pertussis in 92 (68.7%) adolescent, 143 (76.5%) adults, and 72 (88.9%) elderly (p=0.003). The results of MAP showed positive pertussis IgG in adolescent, adults, and elderly were 27 (20.1%), 9 (4.8%), and 3 (3.37%), respectively, which was decreased with age (p<0.001). If MAP positive samples were excluded from the analysis in ELISA positive samples, which express the IgG pertussis due to contact with circulate B, the pertussis IgG positive was 68.9%. In conclusion, there is a correlation between age and percentage of positive IgG pertussis titer in adolescent, adult, and ederly population in Bandung using ELISA and MAP methods. Positive pertussis IgG by ELISA is increased with age.

Keywords

• Bordetella pertussis
• enzyme linked immunosorbent assay
• IgG
• microagglutination