Journal of Veterinary and Animal Sciences (Jan 1998)

CONTROLLED RATE FREEZING OF CANINE SPERMATOZOA1 USING A PROGRAMMABLE FREEZER

  • S.R. Mohanachandran Nair,
  • J. Kalatharan,
  • S.A. Asokan,
  • P. Sridevi,
  • J. Rajasekaran

Journal volume & issue
Vol. 29, no. 1
pp. 36 – 38

Abstract

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A well defined slow freezing rate using a controlled rate freezer was used for cryopreservation of canine spermatozoa using Tris-egg yolk extender. Viability and membrane integrity of spermatozoa were assessed following freez-thawing. The freezing rate of -2 ° C min-' from 5 ° C to -15 ° C and -5 ° C min-' from -15 ° C to -40 ° C and -10 ° C min-' from -40 ° C to -70 ° C resulted in acceptable level of fertile spermatozoa following freez-thawing.