Protective Effect of Vitamin E on Sperm Parameters and Embryological Development after In Vitro Fertilization in Phenylhydrazine-Treated Mice

Abstract

Background and purpose: Oxidative stress is amongst the factors that change the parameters of sperm and embryo development. This study investigated the protective effects of vitamin E on sperm parameters and In Vitro Fertilization (IVF). Materials and methods: In the present study, male mice were divided into four groups to recieve physiological saline (control group), PHZ, vitamin E +PHZ, and vitamin E. Female mice were used for In Vitro Fertilization. Thirty five days after treatments, the male mice were euthanized and their sperm parameters were studied. Stimulating ovule development was done. The animals were euthanized, and then the ova were fertilized. Data analysis was conducted in SPSS applying ANOVA. Results: Compared to the control group, the mean percentage of sperms with damaged DNA, lack of nuclear maturation, and abnormal morphology increased significantly in PHZ group, which was improved by antioxidants (P<0.05). In PHZ group, the mean percentage of fertility, blastocyst, and blocked embryos increased significantly compared to the control group (P<0.05). But considerable improvements were seen in these parameters after receiving vitamin E. Conclusion: Current study showed that vitamin E could improve sperm indicators and embryological development in vitro. Therefore, it is recommended in order to improve the aforementioned parameters.

Keywords

• vitamin e
• phenylhydrazine
• sperm quality
• ivf
• mice