Spheroid Coculture of Human Gingiva-Derived Progenitor Cells With Endothelial Cells in Modified Platelet Lysate Hydrogels

Siddharth Shanbhag; Siddharth Shanbhag; Ahmad Rashad; Ellen Helgeland Nymark; Salwa Suliman; Catharina de Lange Davies; Andreas Stavropoulos; Andreas Stavropoulos; Anne Isine Bolstad; Kamal Mustafa

doi:10.3389/fbioe.2021.739225

Frontiers in Bioengineering and Biotechnology (Aug 2021)

Spheroid Coculture of Human Gingiva-Derived Progenitor Cells With Endothelial Cells in Modified Platelet Lysate Hydrogels

Siddharth Shanbhag,
Siddharth Shanbhag,
Ahmad Rashad,
Ellen Helgeland Nymark,
Salwa Suliman,
Catharina de Lange Davies,
Andreas Stavropoulos,
Andreas Stavropoulos,
Anne Isine Bolstad,
Kamal Mustafa

Affiliations

Siddharth Shanbhag: Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway
Siddharth Shanbhag: Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway
Ahmad Rashad: Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway
Ellen Helgeland Nymark: Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
Salwa Suliman: Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway
Catharina de Lange Davies: Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
Andreas Stavropoulos: Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden
Andreas Stavropoulos: Division of Regenerative Medicine and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland
Anne Isine Bolstad: Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway
Kamal Mustafa: Center for Translational Oral Research (TOR), Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway

DOI: https://doi.org/10.3389/fbioe.2021.739225
Journal volume & issue: Vol. 9

Abstract

Read online

Cell coculture strategies can promote angiogenesis within tissue engineering constructs. This study aimed to test the angiogenic potential of human umbilical vein endothelial cells (HUVEC) cocultured with gingiva-derived progenitor cells (GPC) as spheroids in a xeno-free environment. Human platelet lysate (HPL) was used as a cell culture supplement and as a hydrogel matrix (HPLG) for spheroid encapsulation. HUVEC and HUVEC + GPC (1:1 or 5:1) spheroids were encapsulated in various HPLG formulations. Angiogenesis was assessed via in vitro sprouting and in vivo chick chorioallantoic membrane (CAM) assays. HUVEC revealed characteristic in vitro sprouting in HPL/HPLG and this was significantly enhanced in cocultures with GPC (p < 0.05). A trend for greater sprouting was observed in 5:1 vs 1:1 HUVEC + GPC spheroids and in certain HPLG formulations (p > 0.05). Both HUVEC and HUVEC + GPC spheroids in HPLG revealed abundant and comparable neoangiogenesis in the CAM assay (p > 0.05). Spheroid coculture of HUVEC + GPC in HPLG represents a promising strategy to promote angiogenesis.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

About the journal

Abstract

Keywords