IN VITRO PROPAGATION OF ANGIOPTERIS EVECT A USING SPORES

Abstract

Techniques of e s t a b l i s h i n g Angiopleris evecta plants in vitro were studied. Soaking of A. evecta spores in water for 2 4 h ours mar kedly r edu ced s pore contamination . Soaking of the spores in 1 -2 % of sodi um hy po chlori te for less th an 5 minut es allo wed satisfa ctor y disinfes tation without affe cting spore v i a b i l i t y . Lower concentration of minerals (1/4 MS) , p resence of charcoal in the me dium and exp osure of the spores to l i g h t were cr u c i a l for spore germination an d gainetophytc dev elopment of A. evec ta.

Keywords

• Angiop le ri s evecta I K i n g fern / spore / MS medium / bleach / l i g h t / t issue culture / sporophyte / gamc tophytc