Generation of two induced pluripotent stem cell lines from patients with Down syndrome

Wenjuan Zhu; Wenqiang Liu; Rebecca Yu; Melanie Manning; Anitra Waran Romfh; Joseph C. Wu

Stem Cell Research (Oct 2023)

Generation of two induced pluripotent stem cell lines from patients with Down syndrome

Wenjuan Zhu,
Wenqiang Liu,
Rebecca Yu,
Melanie Manning,
Anitra Waran Romfh,
Joseph C. Wu

Affiliations

Wenjuan Zhu: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Wenqiang Liu: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Rebecca Yu: Greenstone Biosciences, Palo Alto, CA 94304, USA
Melanie Manning: Department of Pathology and Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
Anitra Waran Romfh: Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Pediatrics, Division of Pediatric Cardiology, Stanford University School of Medicine, Stanford, CA 94305, USA
Joseph C. Wu: Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiology, Stanford University School of Medicine, Stanford, CA 94305, USA; Corresponding author at: 265 Campus Drive, G1120B, Stanford, CA 94305, USA.

Journal volume & issue: Vol. 72
p. 103204

Abstract

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Down syndrome (DS) is caused by trisomy of Homo sapiens chromosome 21 (HSA21) and is by far the most common chromosomal disorder accompanied by neurodevelopmental disorders and congenital heart disease. Here, we generated two induced pluripotent stem cell (iPSC) lines from two patients with DS. These two lines exhibited normal morphology, trisomy 21 karyotype, pluripotency and differentiation capability into derivatives of three germ layers. The patient-specific iPSC lines are an invaluable resource in research to model DS-related cellular and molecular pathologies and test possible therapeutic strategies for DS.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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