Isolation and identification of anti-colorectal cancer peptides from walnut proteins and associated in silico analysis

Jing Xie; ZiShan Hong; JingJing Dai; Tingting Li; Yuying Bai; Li Zhang; Xia Hu; Jinlian Chen; Jun Sheng; Yang Tian

Journal of Functional Foods (Jan 2024)

Isolation and identification of anti-colorectal cancer peptides from walnut proteins and associated in silico analysis

Jing Xie,
ZiShan Hong,
JingJing Dai,
Tingting Li,
Yuying Bai,
Li Zhang,
Xia Hu,
Jinlian Chen,
Jun Sheng,
Yang Tian

Affiliations

Jing Xie: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China
ZiShan Hong: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China
JingJing Dai: School of Tea and Coffee, Pu'er University, Puer 665000, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food, Yunnan Agricultural University, Kunming 650201, PR China
Tingting Li: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China
Yuying Bai: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food, Yunnan Agricultural University, Kunming 650201, PR China
Li Zhang: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food, Yunnan Agricultural University, Kunming 650201, PR China
Xia Hu: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China
Jinlian Chen: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food, Yunnan Agricultural University, Kunming 650201, PR China
Jun Sheng: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, PR China; Corresponding authors at: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China (Jun Sheng). School of Tea and Coffee,Puer University , Puer 665000, PR China (Yang Tian).
Yang Tian: School of Tea and Coffee, Pu'er University, Puer 665000, PR China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, PR China; National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming 650201, PR China; Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, PR China; Corresponding authors at: College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, PR China (Jun Sheng). School of Tea and Coffee,Puer University , Puer 665000, PR China (Yang Tian).

Journal volume & issue: Vol. 112
p. 105952

Abstract

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Walnut protein-derived peptides have a variety of biological activities, but it is unclear whether walnut peptides have anti-colorectal cancer activity. Therefore, the aim of this study was to explore the anti-colorectal cancer peptides of walnut and the underlying mechanism of action by in silico analysis and in vitro evaluation. Walnut protein alcalase hydrolysate (WPH-Al) significantly inhibited the growth and induced the apoptosis of colon cancer HCT116 cells. The < 1 kDa molecular weight fraction (WPH-M1) exhibited more significant inhibition of HCT116 cell proliferation and induction of apoptosis than other fractions. In silico analysis identified three peptides (PISLKSE, VSLP, and SHTLP) in WPH-M1 with potential anticancer activity. Network pharmacology analysis further identified 72 targets of these peptides associated with colorectal cancer, which were mainly enriched in pathways related to cancer and apoptosis, among which caspase-3 (CASP3) and metalloproteinase-9 (MMP9) were identified as candidate core targets. These results were verified by molecular docking. Overall, these screened walnut-derived peptides may represent a promising functional food with anti-colorectal cancer effects.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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