A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

Anupriya M. Geethakumari; Wesam S. Ahmed; Saad Rasool; Asma Fatima; S. M. Nasir Uddin; Mustapha Aouida; Kabir H. Biswas

doi:10.1038/s42004-022-00731-2

Communications Chemistry (Sep 2022)

A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor

Anupriya M. Geethakumari,
Wesam S. Ahmed,
Saad Rasool,
Asma Fatima,
S. M. Nasir Uddin,
Mustapha Aouida,
Kabir H. Biswas

Affiliations

Anupriya M. Geethakumari: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
Wesam S. Ahmed: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
Saad Rasool: Division of Genomics and Precision Medicine, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
Asma Fatima: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
S. M. Nasir Uddin: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
Mustapha Aouida: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation
Kabir H. Biswas: Division of Biological and Biomedical Sciences, College of Health & Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation

DOI: https://doi.org/10.1038/s42004-022-00731-2
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

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The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen and NanoLuc proteins.

Published in Communications Chemistry

ISSN: 2399-3669 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry
Website: https://www.nature.com/commschem

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