Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Yaowei Xuan; Yaowei Xuan; Lin Li; Lin Li; Muzhi Ma; Junkai Cao; Zhen Zhang

doi:10.3389/fbioe.2021.781268

Frontiers in Bioengineering and Biotechnology (Jan 2022)

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Yaowei Xuan,
Yaowei Xuan,
Lin Li,
Lin Li,
Muzhi Ma,
Junkai Cao,
Zhen Zhang

Affiliations

Yaowei Xuan: Medical School of Chinese PLA, Beijing, China
Yaowei Xuan: Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China
Lin Li: Medical School of Chinese PLA, Beijing, China
Lin Li: Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China
Muzhi Ma: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China
Junkai Cao: Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China
Zhen Zhang: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China

DOI: https://doi.org/10.3389/fbioe.2021.781268
Journal volume & issue: Vol. 9

Abstract

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Mineralized collagen has been introduced as a promising barrier membrane material for guided bone regeneration (GBR) due to its biomimetic nanostructure. Immune interaction between materials and host significantly influences the outcome of GBR. However, current barrier membranes are insufficient for clinical application due to limited mechanical or osteoimmunomodulatory properties. In this study, we fabricated hierarchical intrafibrillarly mineralized collagen (HIMC) membrane, comparing with collagen (COL) and extrafibrillarly mineralized collagen (EMC) membranes, HIMC membrane exhibited preferable physicochemical properties by mimicking the nanostructure of natural bone. Bone marrow mesenchymal stem cells (BMSCs) seeded on HIMC membrane showed superior proliferation, adhesion, and osteogenic differentiation capacity. HIMC membrane induced CD206+Arg-1+ M2 macrophage polarization, which in turn promoted more BMSCs migration. In rat skull defects, HIMC membrane promoted the regeneration of new bone with more bone mass and more mature bone architecture. The expression levels of Runx2 and osterix and CD68 + CD206 + M2 macrophage polarization were significantly enhanced. HIMC membrane provides an appropriate osteoimmune microenvironment to promote GBR and represents a promising material for further clinical application.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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