Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications

Robert Hearnden; Balraj Sandhar; Vishal Vyas; M. Paula Longhi

STAR Protocols (Jun 2021)

Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications

Robert Hearnden,
Balraj Sandhar,
Vishal Vyas,
M. Paula Longhi

Affiliations

Robert Hearnden: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK; Barts Cancer Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK
Balraj Sandhar: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK
Vishal Vyas: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK; Department of Cardiology, St Bartholomew’s Hospital, London EC1A 7BE, UK
M. Paula Longhi: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100422

Abstract

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Summary: This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations.For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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