Label-free Raman imaging for screening of anti-inflammatory function food

Qi Zeng; Yangyao Peng; Xianzhen Zhou; Jiaojiao Zhang; Yuhang Yang; Xinyi Xu; Bin Guan; Yuntian Zhang; Xiaojia Hu; Xueli Chen

Food Chemistry: X (Jun 2024)

Label-free Raman imaging for screening of anti-inflammatory function food

Qi Zeng,
Yangyao Peng,
Xianzhen Zhou,
Jiaojiao Zhang,
Yuhang Yang,
Xinyi Xu,
Bin Guan,
Yuntian Zhang,
Xiaojia Hu,
Xueli Chen

Affiliations

Qi Zeng: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, China
Yangyao Peng: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China
Xianzhen Zhou: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China
Jiaojiao Zhang: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China
Yuhang Yang: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China
Xinyi Xu: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, China
Bin Guan: Affiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi Fifth People's Hospital, Wuxi 214000, China
Yuntian Zhang: Shanghai Nature's Sunshine Health Products Co. Ltd, Shanghai 200040, China
Xiaojia Hu: Shanghai Nature's Sunshine Health Products Co. Ltd, Shanghai 200040, China; Corresponding author.
Xueli Chen: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, China; Corresponding author at: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China.

Journal volume & issue: Vol. 22
p. 101297

Abstract

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Natural bioactive compounds and plant constituents are considered to have a positive anti-inflammatory effect. This study aimed to establish a screening technique for anti-inflammatory function in foods based on label-free Raman imaging. A visible anti-inflammatory analysis method based on coherent anti-Stokes Raman scattering (CARS) was established with an LPS-induced RAW264.7 cell model. Dynamic changes in proteins and lipids were determined at laser pump light wavelengths of 2956 cm−1 and 2856 cm−1, respectively. The method was applied to a plant-based formula (JC) with anti-inflammatory activity. Q-TOF-MS and HPLC analyses revealed the main active constituents of JC as quercetin, kaempferol, l-glutamine, and sodium copper chlorophyllin. In in vitro and in vivo verification experiments, JC showed significant anti-inflammatory activity by regulating the TLR4/NF-κB pathway. In conclusion, this study successfully established a label-free and visible method for screening anti-inflammatory constituents in plant-based food products, which will facilitate the evaluation of functional foods.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

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