Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

Andreja Jungić; Vladimir Savić; Josip Madić; Ljubo Barbić; Besi Roić; Dragan Brnić; Jelena Prpić; Lorena Jemeršić; Dinko Novosel

doi:10.3390/pathogens10111527

Pathogens (Nov 2021)

Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

Andreja Jungić,
Vladimir Savić,
Josip Madić,
Ljubo Barbić,
Besi Roić,
Dragan Brnić,
Jelena Prpić,
Lorena Jemeršić,
Dinko Novosel

Affiliations

Andreja Jungić: Department of Virology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia
Vladimir Savić: Poultry Center, Croatian Veterinary Institute, Heinzelova 55, 10000 Zagreb, Croatia
Josip Madić: Deparment of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
Ljubo Barbić: Deparment of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia
Besi Roić: Department of Virology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia
Dragan Brnić: Department of Virology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia
Jelena Prpić: Department of Virology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia
Lorena Jemeršić: Department of Virology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia
Dinko Novosel: Department of Pathology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia

DOI: https://doi.org/10.3390/pathogens10111527
Journal volume & issue: Vol. 10, no. 11
p. 1527

Abstract

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In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.

Published in Pathogens

ISSN: 2076-0817 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/pathogens

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