Diabetes, Metabolic Syndrome and Obesity (Aug 2022)
Association of lncRNA LINC01173 Expression with Vitamin-D and Vitamin B12 Level Among Type 2 Diabetes Patients
Abstract
Alanoud Aladel,1,* Amit K Verma,2,* Sadaf Dabeer,3 Irfan Ahmad,4 Mohammad Y Alshahrani,4 Mohammad S AboHassan,4 Mohammad Idreesh Khan,5,* Malak Ghazi Almutairi,6 Mirza Masroor Ali Beg7,8 1Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia; 2Department of Biotechnology, Jamia Millia Islamia, New Delhi, India; 3Division of Endocrinology Metabolism, and Lipids, School of Medicine, Emory University, Atlanta, GA, USA; 4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia; 5Department of Clinical Nutrition, College of Applied Health Sciences in Arras, Qassim University, Buraidah, Saudi Arabia; 6Department of Nutrition, Almethnab General Hospital, Qassim Health Cluster, Ministry of Health, Al Mithnab, Saudi Arabia; 7Faculty of Medicine, Alatoo International University, Bishkek, Kyrgyzstan; 8Centre for Promotion of Medical Research, Bishkek, Kyrgyzstan*These authors contributed equally to this workCorrespondence: Mirza Masroor Ali Beg, Email [email protected]: Type 2 diabetes mellitus (T2DM) has risen to become the world’s most serious public health problem in recent years, and the role of long noncoding RNAs (lncRNAs) in the onset and progression of T2DM, as well as special attention to vitamins, has gotten a lot of attention recently.Methods: The aim of the study was to analyze lncRNA LINC01173 expression along with assessment of vitamin-D and B12 among the T2DM cases. Quantitative RT-PCR was used to analyze the expression of lncRNA LINC01173. Vitamin-D and B12 were analyzed by chemiluminescence-based assay.Results: The present study observed that the T2DM cases had 6.67-fold increased lncRNA LINC01173 expression compared to healthy controls. Expression of lncRNA LINC01173 was found to be associated with hypertension (p=0.03), wound healing (p=0.04), and blurred vision (p< 0.0001). It was observed that the T2DM cases with vitamin-D deficiency had a significant association with fasting glucose level (p=0.01) and HbA1C level (p=0.01) among the T2DM cases. The association of lncRNA LINC01173 with vitamin-D was analyzed and it was observed that the vitamin-D deficient cases had higher lncRNA LINC01173 expression compared to insufficient T2DM cases (p=0.01) and sufficient T2DM cases (p=0.0006). It was also observed that the T2DM cases with smoking had a 8.33-fold lncRNA LINC01173 expression while non-smokers had a 5.43-fold lncRNA LINC01173 expression (p< 0.0001).Conclusion: The study concluded that the increased lncRNA LINC01173 expression was observed to be linked with alteration in vitamin-D level and smoking habit. Altered expression of lncRNA LINC01173 expression was linked with fasting glucose and HbA1C alteration. Collectively, lncRNA LINC01173 expression, vitamin-D alteration, as well as smoking habit may cause the disease severity and increase the pathogenesis of disease.Keywords: long noncoding RNA, vitamin-D, vitamin-B12, diabetes
