Physiological Responses Revealed Static Magnetic Fields Potentially Improving the Tolerance of Poplar Seedlings to Salt Stress

Abstract

Magnetic fields play an important role in regulating plant growth and development, especially in improving plant stress tolerance. However, the physiological mechanism underlying the magnetic effects is still unclear. Here, we examined changes in reactive oxygen species (ROS) levels and ion flux in poplar (Populus × deltoides ‘Lulin-2’) seedling roots under salt stress in a static magnetic field (SMF). SMF treatment significantly increased seedling growth and mitigated the effects of salt stress on root growth. Furthermore, SMF treatment activated ROS and calcium signals in poplar roots. Relative to the SMF treatment group, control plants had significantly higher levels of cytoplasmic free Ca2+ ([Ca2+]cyt) and ROS following exposure to high salt concentrations. Under salt conditions, SMF treatment reduced increases in Na+ concentrations and maintained stable K+ and Ca2+ concentrations and K+/Na+ and Ca2+/Na+ ratios. NMT analysis suggests that SMF treatment may drive cation effluxes in poplar seedling roots. Susceptibility tests of Na+-transport inhibitors indicated that SMF treatment contributed to Na+ repulsion and H+ uptake under salt stress. Moreover, SMF exposure allowed roots to retain the ability to reduce salt-induced K+ and Ca2+ root effluxes, and qRT-PCR results demonstrate that SMF treatment can increase the expression of stress-responsive genes such as PtrRBOHF, PtrNHX1 and PtrHA5 in poplar seedlings. Therefore, we conclude that treating poplar seedlings with SMF can help them establish a stable tolerance to salt stress by regulating ROS, [Ca2+]cyt, and their regulatory networks. This study examined the physiological responses of poplar to SMF exposure under salt stress, providing insights into plant magnetobiological effects.

Keywords

• magnetobiological effects
• reactive oxygen species
• intracellular ion homeostasis
• salinity tolerance