Formulation and Evaluation of Sugarcane-Bagasse-Based Biocontrol Agents for Sustainable Phytopathogen Management

Abstract

Biocontrol agents are microbiological-based alternatives to agrochemicals due to their effective and sustainable attributes in controlling phytopathogens. This research highlights the formulation of biocontrol agents using sugarcane-bagasse as a carrier matrix and the evaluation of the formulants in phytopathogen management. The isolated rhizospheric bacteria were screened for the antibiosis trait responsible for biocontrol activity using the agar streak method. Bacterial isolates with antibiosis potential were further identified phenotypically. The carrier was prepared by oven drying the sugarcane-bagasse at 90 °C for three days while grinding and sieving using a mesh sieve of 1.16 mm was done afterwards. For the biocontrol formulation, 200 mL of biocontrol inoculum was added to 20 g of sugarcane-bagasse for each organism to form the final products. Water and adhesion capacities were conducted on the three formulations and, the antagonistic potential of the formulants were evaluated using the maize growth profile after 21 days. A total of nine isolates were obtained; only three (3) showed antibiosis antagonistic activity and were further utilized for the formulations branded ZEEMYC (Mycobacterium spp.), ZEEPAS (Pseudomonas spp.), and ZEEBAC (Bacillus spp.), respectively. The water capacities of the three formulations were between 6.9 g and 9.9 g, respectively, while adhesion capacity was also observed. On day five (5), maize seeds planted in all pots sprouted, except diseased seeds without a biocontrol agent (DSs). On day 11, plant height, shoot length, and root length ranged between 36.5 cm and 39 cm, 31 cm and 34 cm, and 5 cm and 7 cm for plants with a biocontrol agent. Those of the control (healthy seeds without biocontrol) were 42 cm, 34.5 cm, and 7.5 cm, while barely visible growth was observed in the DSs. This study displays the potential of natural-based biocontrol agents in controlling the phytopathogen Aspergillus niger and contributes significantly to SDG 2.

Keywords

• biocontrol
• plant growth promoting rhizobacteria
• bioformulation
• microbial inoculants
• sugarcane bagasse
• antibiosis