Characterization of Soybean Events with Enhanced Expression of the Microtubule-Associated Protein 65-1 (MAP65-1)

Panya Kim; Samira Mahboob; Hanh T. Nguyen; Samuel Eastman; Olivia Fiala; Matthew Sousek; Roch E. Gaussoin; Jae L. Brungardt; Tamra A. Jackson-Ziems; Rebecca Roston; James R. Alfano; Tom Elmo Clemente; Ming Guo

doi:10.1094/MPMI-09-23-0134-R

Molecular Plant-Microbe Interactions (Jan 2024)

Characterization of Soybean Events with Enhanced Expression of the Microtubule-Associated Protein 65-1 (MAP65-1)

Panya Kim,
Samira Mahboob,
Hanh T. Nguyen,
Samuel Eastman,
Olivia Fiala,
Matthew Sousek,
Roch E. Gaussoin,
Jae L. Brungardt,
Tamra A. Jackson-Ziems,
Rebecca Roston,
James R. Alfano,
Tom Elmo Clemente,
Ming Guo

Affiliations

Panya Kim: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
Samira Mahboob: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
Hanh T. Nguyen: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Samuel Eastman: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
Olivia Fiala: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Matthew Sousek: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Roch E. Gaussoin: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Jae L. Brungardt: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
Tamra A. Jackson-Ziems: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
Rebecca Roston: Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A.
James R. Alfano: Center for Plant Science Innovation and Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68588, U.S.A. (deceased)
Tom Elmo Clemente: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Ming Guo: Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.

DOI: https://doi.org/10.1094/MPMI-09-23-0134-R
Journal volume & issue: Vol. 37, no. 1
pp. 62 – 71

Abstract

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Microtubule-associated protein 65-1 (MAP65-1) protein plays an essential role in plant cellular dynamics through impacting stabilization of the cytoskeleton by serving as a crosslinker of microtubules. The role of MAP65-1 in plants has been associated with phenotypic outcomes in response to various environmental stresses. The Arabidopsis MAP65-1 (AtMAP65-1) is a known virulence target of plant bacterial pathogens and is thus a component of plant immunity. Soybean events were generated that carry transgenic alleles for both AtMAP65-1 and GmMAP65-1, the soybean AtMAP65-1 homolog, under control of cauliflower mosaic virus 35S promoter. Both AtMAP65-1 and GmMAP65-1 transgenic soybeans are more resistant to challenges by the soybean bacterial pathogen Pseudomonas syringae pv. glycinea and the oomycete pathogen Phytophthora sojae, but not the soybean cyst nematode, Heterodera glycines. Soybean plants expressing AtMAP65-1 and GmMAP65-1 also display a tolerance to the herbicide oryzalin, which has a mode of action to destabilize microtubules. In addition, GmMAP65-1-expressing soybean plants show reduced cytosol ion leakage under freezing conditions, hinting that ectopic expression of GmMAP65-1 may enhance cold tolerance in soybean. Taken together, overexpression of AtMAP65-1 and GmMAP65-1 confers tolerance of soybean plants to various biotic and abiotic stresses. [Graphic: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Published in Molecular Plant-Microbe Interactions

ISSN: 0894-0282 (Print); 1943-7706 (Online)
Publisher: The American Phytopathological Society
Country of publisher: United States
LCC subjects: Science: Microbiology; Science: Botany
Website: https://apsjournals.apsnet.org/journal/mpmi

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