Structural remodelling of the carbon–phosphorus lyase machinery by a dual ABC ATPase

Søren K. Amstrup; Sui Ching Ong; Nicholas Sofos; Jesper L. Karlsen; Ragnhild B. Skjerning; Thomas Boesen; Jan J. Enghild; Bjarne Hove-Jensen; Ditlev E. Brodersen

doi:10.1038/s41467-023-36604-y

Nature Communications (Feb 2023)

Structural remodelling of the carbon–phosphorus lyase machinery by a dual ABC ATPase

Søren K. Amstrup,
Sui Ching Ong,
Nicholas Sofos,
Jesper L. Karlsen,
Ragnhild B. Skjerning,
Thomas Boesen,
Jan J. Enghild,
Bjarne Hove-Jensen,
Ditlev E. Brodersen

Affiliations

Søren K. Amstrup: Department of Molecular Biology and Genetics, Aarhus University
Sui Ching Ong: Department of Molecular Biology and Genetics, Aarhus University
Nicholas Sofos: Department of Molecular Biology and Genetics, Aarhus University
Jesper L. Karlsen: Department of Molecular Biology and Genetics, Aarhus University
Ragnhild B. Skjerning: Department of Molecular Biology and Genetics, Aarhus University
Thomas Boesen: Interdisciplinary Nanoscience Centre (iNANO) Aarhus University
Jan J. Enghild: Department of Molecular Biology and Genetics, Aarhus University
Bjarne Hove-Jensen: Department of Molecular Biology and Genetics, Aarhus University
Ditlev E. Brodersen: Department of Molecular Biology and Genetics, Aarhus University

DOI: https://doi.org/10.1038/s41467-023-36604-y
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 12

Abstract

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Here, authors analyse the structural organisation of the large carbon-phosphorus lyase enzyme from bacteria using electron microscopy and discover that it contains two ATP-binding cassette dimers of PhnK and PhnL and opens upon ATP hydrolysis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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