Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme

Jiangyu Yan; Thomas R. Beattie; Adriana L. Rojas; Kelly Schermerhorn; Tamzin Gristwood; Jonathan C. Trinidad; Sonja V. Albers; Pietro Roversi; Andrew F. Gardner; Nicola G. A. Abrescia; Stephen D. Bell

doi:10.1038/ncomms15075

Nature Communications (May 2017)

Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme

Jiangyu Yan,
Thomas R. Beattie,
Adriana L. Rojas,
Kelly Schermerhorn,
Tamzin Gristwood,
Jonathan C. Trinidad,
Sonja V. Albers,
Pietro Roversi,
Andrew F. Gardner,
Nicola G. A. Abrescia,
Stephen D. Bell

Affiliations

Jiangyu Yan: Department of Molecular and Cellular Biochemistry, Indiana University
Thomas R. Beattie: Sir William Dunn School of Pathology, University of Oxford
Adriana L. Rojas: Structural Biology Unit, CIC bioGUNE, CIBERehd
Kelly Schermerhorn: New England Biolabs
Tamzin Gristwood: Sir William Dunn School of Pathology, University of Oxford
Jonathan C. Trinidad: Department of Chemistry, Indiana University
Sonja V. Albers: Molecular Biology of Archaea, Institute of Biology II, Microbiology, University of Freiburg
Pietro Roversi: Structural Biology Unit, CIC bioGUNE, CIBERehd
Andrew F. Gardner: New England Biolabs
Nicola G. A. Abrescia: Structural Biology Unit, CIC bioGUNE, CIBERehd
Stephen D. Bell: Department of Molecular and Cellular Biochemistry, Indiana University

DOI: https://doi.org/10.1038/ncomms15075
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 15

Abstract

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The current model for B-family DNA polymerases in archaea is one of single-subunit enzymes in contrast to the multi-subunit complexes in eukaryotes. Here the authors show that PolB1 fromSulfolobus solfataricusexists as a heterotrimeric complex in cell extracts.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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