CHARACTERISTICS OF FORMATION, INHIBITION AND DESTRUCTION OF YERSINIA PSEUDOTUBERCULOSIS BIOFILMS FORMING ON ABIOTIC SURFACES

Abstract

Aim. Detection of conditions of Yersinia pseudo tuberculosis biofilm formation, their quantitative testing. Materials and methods. Y. pseudotuberculosis strains, nutrient media, standard 96-well polystyrene plates, crystal violet dye as well as bacteriologic, spectrophotometric, statistical methods were used. Results. All the studied Y. pseudotuberculosis strains formed a well expressed biofilm on abiotic surface during cultivation of bacteria in 200 pl of a plate well at a temperature of 20 - 22°C for 4 - 7 days. Bacteria CFU number in biofilm reduced by day 10 of incubation. DNAse I was found to inhibit biofilm formation, and also partially destroyed mature Y. pseudotuberculosis biofilm. The presence of DNA in extra-cellular matrix of biofilm was shown. Conclusion. An ability of Y. pseudotuberculosis to form biofilm on abiotic surface was established. The conditions of biofilm formation were determined. Inhibiting effect of DNAse I on Y. pseudotuberculosis was shown.

Keywords

• yersinia pseudotuberculosis
• yersinia pseudotuberculosis
• biofilm
• dnase i