Study of Pathogenicity Test, Antifungal Activity, and Secondary Metabolites of Bacillus spp. from Lake Bogoria as Biocontrol of Rhizoctonia solani Kühn in Phaseolus vulgaris L.

Abstract

The common bean (Phaseolus vulgaris L.) is a yearly herbaceous plant grown for its edible dry seeds. Despite that, pests and diseases have contributed to the decline of common bean production in Kenya. Therefore, the study aimed to identify bacteria from Lake Bogoria, assess the pathogenicity of Rhizoctonia solani Kühn, screen for effective antifungal agents, and determine secondary metabolites for the biocontrol of R. solani. A total of 49 bacteria were isolated, of which 10 isolates had varied mycelial inhibition rates of R. solani in the co-culture technique. The efficacy of volatile compounds of the three selected bacterial strains had varied mycelial growth and percent reduction against R. solani. The pathogenicity assay showed varied plant parameters and biomass of R. solani on common bean plantlets. The molecular characterization based on 16 S ribosomal RNA confirmed the selected bacterial strains’ identity with a diversity similar to the Bacillus genus. Gas chromatography-mass spectrometry analysis of secondary metabolites showed different antimicrobial compounds produced by Bacillus subtilis strain TW21. In conclusion, Lake Bogoria harbors useful microbes as biocontrol agents against plant pathogens. The current study discovers the potential biocontrol bacteria isolates from Lake Bogoria as alternative bioagents against R. solani. Therefore, the isolate Bacillus subtilis strain TW21 can be assessed further for toxicological and ecotoxicological studies and registered by the Pest Control Products Board (PCPB), Kenya, as a biocontrol product against common diseases affecting common beans’ production.