Fixed-target serial oscillation crystallography at room temperature

Jennifer L. Wierman; Olivier Paré-Labrosse; Antoine Sarracini; Jessica E. Besaw; Michael J. Cook; Saeed Oghbaey; Hazem Daoud; Pedram Mehrabi; Irina Kriksunov; Anling Kuo; David J. Schuller; Scott Smith; Oliver P. Ernst; Doletha M. E. Szebenyi; Sol M. Gruner; R. J. Dwayne Miller; Aaron D. Finke

doi:10.1107/S2052252519001453

IUCrJ (Mar 2019)

Fixed-target serial oscillation crystallography at room temperature

Jennifer L. Wierman,
Olivier Paré-Labrosse,
Antoine Sarracini,
Jessica E. Besaw,
Michael J. Cook,
Saeed Oghbaey,
Hazem Daoud,
Pedram Mehrabi,
Irina Kriksunov,
Anling Kuo,
David J. Schuller,
Scott Smith,
Oliver P. Ernst,
Doletha M. E. Szebenyi,
Sol M. Gruner,
R. J. Dwayne Miller,
Aaron D. Finke

Affiliations

Jennifer L. Wierman: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Olivier Paré-Labrosse: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Antoine Sarracini: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Jessica E. Besaw: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Michael J. Cook: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Saeed Oghbaey: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Hazem Daoud: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Pedram Mehrabi: Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany
Irina Kriksunov: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Anling Kuo: Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
David J. Schuller: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Scott Smith: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Oliver P. Ernst: Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
Doletha M. E. Szebenyi: MacCHESS, Cornell University, Ithaca, NY 14853, USA
Sol M. Gruner: MacCHESS, Cornell University, Ithaca, NY 14853, USA
R. J. Dwayne Miller: Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada
Aaron D. Finke: MacCHESS, Cornell University, Ithaca, NY 14853, USA

DOI: https://doi.org/10.1107/S2052252519001453
Journal volume & issue: Vol. 6, no. 2
pp. 305 – 316

Abstract

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A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1–5° of data per crystal at room temperature with fast (10° s−1) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement – up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.

Published in IUCrJ

ISSN: 2052-2525 (Online)
Publisher: International Union of Crystallography
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Crystallography
Website: https://journals.iucr.org/m/

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