Neurobiology of Disease (Jul 1998)
Calcium Responses in Fibroblasts from Asymptomatic Members of Alzheimer's Disease Families
- René Etcheberrigaray,
- Naohide Hirashima,
- Linda Nee,
- José Prince,
- Stefano Govoni,
- Marco Racchi,
- Rudolph E. Tanzi,
- Daniel L. Alkon
Affiliations
- René Etcheberrigaray
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Naohide Hirashima
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Linda Nee
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- José Prince
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Stefano Govoni
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Marco Racchi
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Rudolph E. Tanzi
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Daniel L. Alkon
- Laboratory of Applied Neuroscience, Institute for Cognitive and Computational Sciences, Georgetown University Medical Center, The Research Building, WP14, 3970 Reservoir Road, NW Washington, DC, 20007; Laboratory of Adaptive Systems, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Family Studies Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892; Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, 02129; Institute of Pharmacology, University of Pavia, Pavia, Italy; Laboratory of Molecular and Cellular Neurobiology, Alzheimer's Department, Sacred Heart–FBF Hospital of Brescia, Brescia, Italy
- Journal volume & issue
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Vol. 5,
no. 1
pp. 37 – 45
Abstract
We have previously identified alterations of K+channel function, IP3-mediated calcium release, and Cp20 (a memory-associated GTP binding protein) in fibroblasts from Alzheimer's disease (AD) patients vs controls. Some of these alterations can be integrated into an index that distinguishes AD patients from controls with both high specificity and high sensitivity. We report here that alterations in IP3-mediated calcium responses are present in a large proportion of AD family members (i.e., individuals at high risk) before clinical symptoms of Alzheimer's disease are present. This was not the case if such members later “escaped” AD symptoms. This preclinical calcium signal correlate of later AD does not reflect, however, the presence of the PS1 familial AD gene.