Nukleonika (Dec 2015)
Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy
- Kubicz Ewelina,
- Jasińska Bożena,
- Zgardzińska Bożena,
- Bednarski Tomasz,
- Białas Piotr,
- Czerwiński Eryk,
- Gajos Aleksander,
- Gorgol Marek,
- Kamińska Daria,
- Kapłon Łukasz,
- Kochanowski Andrzej,
- Korcyl Grzegorz,
- Kowalski Paweł,
- Kozik Tomasz,
- Krzemień Wojciech,
- Niedźwiecki Szymon,
- Pałka Marek,
- Raczyński Lech,
- Rajfur Zenon,
- Rudy Zbigniew,
- Rundel Oleksandr,
- Sharma Neha Gupta,
- Silarski Michał,
- Słomski Artur,
- Strzelecki Adam,
- Wieczorek Anna,
- Wiślicki Wojciech,
- Zieliński Marcin,
- Moskal Paweł
Affiliations
- Kubicz Ewelina
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Jasińska Bożena
- Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland
- Zgardzińska Bożena
- Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland
- Bednarski Tomasz
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Białas Piotr
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Czerwiński Eryk
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Gajos Aleksander
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Gorgol Marek
- Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland
- Kamińska Daria
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Kapłon Łukasz
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland and Institute of Metallurgy and Materials Science PAS, 25 W. Reymonta Str., 30-059 Kraków, Poland
- Kochanowski Andrzej
- Faculty of Chemistry, Department of Chemical Technology, Jagiellonian University, 3 R. Ingardena Str., 30-059 Kraków, Poland
- Korcyl Grzegorz
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Kowalski Paweł
- Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland
- Kozik Tomasz
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Krzemień Wojciech
- High Energy Physics Division, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland
- Niedźwiecki Szymon
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Pałka Marek
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Raczyński Lech
- Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland
- Rajfur Zenon
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Rudy Zbigniew
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Rundel Oleksandr
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Sharma Neha Gupta
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Silarski Michał
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Słomski Artur
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Strzelecki Adam
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Wieczorek Anna
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland and Institute of Metallurgy and Materials Science PAS, 25 W. Reymonta Str., 30-059 Kraków, Poland
- Wiślicki Wojciech
- Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland
- Zieliński Marcin
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- Moskal Paweł
- Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
- DOI
- https://doi.org/10.1515/nuka-2015-0135
- Journal volume & issue
-
Vol. 60,
no. 4
pp. 749 – 753
Abstract
Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.
Keywords
- environmental scanning electron microscopy (esem)
- free volume
- inverted microscopy
- positron annihilation
- positronium
- yeasts
