Correlationship between total proteins SUMOylation and papillary thyroid carcinoma in males

Abstract

Objective To investigate the relationship between protein SUMOylation level and the prognosis of papillary thyroid carcinoma(PTC) in males. Methods Protein SUMOylation levels in PTC was analyzed by bioinformatics based on GTEx and TCGA databases and validated by immunohistochemical staining and Western blotting in our clinical pairs specimens. The mRNA expression of the protein SUMOylation associated genes were measured by fluorescent quantitative real-time polymerase chain reaction (qRT-PCR) in surgical pairs specimens. Results The expression level of SUMOylation in the tumor tissues of PTC showed an elevated trend（P<0.05）, and was associated with poor prognosis of the patients by TCGA and GTEx databases analysis(P=0.021）. In the clinical samples of our hospital, it was verified that the level of SUMOylation in tumor tissues was higher than that of the paired non-tumor tissues（P<0.05）. However, qRT-PCR showed no significant changes in the transcriptional level of the protein SUMOylation associated genes in most cases. Conclusions Protein SUMOylation in thyroid tumor tissues were higher than that in paired non-tumor tissues, and the higher SUMOylation levels levels in tumor tissues were, the shorter overall survival time of the patients was.

Keywords

• |sumoylation|males|papillary thyroid carcinoma (ptc)