Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor

Michael H. Berry; Amy Holt; Joshua Levitz; Johannes Broichhagen; Benjamin M. Gaub; Meike Visel; Cherise Stanley; Krishan Aghi; Yang Joon Kim; Kevin Cao; Richard H. Kramer; Dirk Trauner; John Flannery; Ehud Y. Isacoff

doi:10.1038/s41467-017-01990-7

Nature Communications (Nov 2017)

Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor

Michael H. Berry,
Amy Holt,
Joshua Levitz,
Johannes Broichhagen,
Benjamin M. Gaub,
Meike Visel,
Cherise Stanley,
Krishan Aghi,
Yang Joon Kim,
Kevin Cao,
Richard H. Kramer,
Dirk Trauner,
John Flannery,
Ehud Y. Isacoff

Affiliations

Michael H. Berry: Department of Molecular and Cell Biology, University of California
Amy Holt: Department of Molecular and Cell Biology, University of California
Joshua Levitz: Department of Molecular and Cell Biology, University of California
Johannes Broichhagen: Department of Chemistry, Ludwig-Maximilians-Universität München, and Munich Center for Integrated Protein Science, Butenandtstrasse 5-13
Benjamin M. Gaub: Department of Molecular and Cell Biology, University of California
Meike Visel: Department of Molecular and Cell Biology, University of California
Cherise Stanley: Department of Molecular and Cell Biology, University of California
Krishan Aghi: Helen Wills Neuroscience Institute, University of California
Yang Joon Kim: Biophysics Graduate Program, University of California
Kevin Cao: Department of Molecular and Cell Biology, University of California
Richard H. Kramer: Department of Molecular and Cell Biology, University of California
Dirk Trauner: Department of Chemistry, Ludwig-Maximilians-Universität München, and Munich Center for Integrated Protein Science, Butenandtstrasse 5-13
John Flannery: Department of Molecular and Cell Biology, University of California
Ehud Y. Isacoff: Department of Molecular and Cell Biology, University of California

DOI: https://doi.org/10.1038/s41467-017-01990-7
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

Read online

To restore sight after retinal degeneration, one approach is to express light-sensitive proteins in remaining cells. Here the authors combine a light-sensitive engineered G protein-coupled receptor and ion channels to restore ON and OFF responses as well as superior visual pattern discrimination.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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