Effects of insecticidal proteins of Enterobacter cloacae NK on cellular immunity of Galleria mellonella larvae

Chunli Liao; Chunli Liao; Chunli Liao; Ran Huang; Yi Yang; Yapeng Huang; Kai Zhang; Liang Ma; Taotao Li; Taotao Li; Taotao Li; Lianzhe Wang; Lianzhe Wang; Lianzhe Wang; Huamin Zhang; Huamin Zhang; Huamin Zhang; Bingbing Li; Bingbing Li; Bingbing Li

doi:10.3389/fmicb.2023.1154811

Frontiers in Microbiology (May 2023)

Effects of insecticidal proteins of Enterobacter cloacae NK on cellular immunity of Galleria mellonella larvae

Chunli Liao,
Chunli Liao,
Chunli Liao,
Ran Huang,
Yi Yang,
Yapeng Huang,
Kai Zhang,
Liang Ma,
Taotao Li,
Taotao Li,
Taotao Li,
Lianzhe Wang,
Lianzhe Wang,
Lianzhe Wang,
Huamin Zhang,
Huamin Zhang,
Huamin Zhang,
Bingbing Li,
Bingbing Li,
Bingbing Li

Affiliations

Chunli Liao: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Chunli Liao: Center of Healthy Food Engineering and Technology of Henan, Henan University of Urban Construction, Pingdingshan, Henan, China
Chunli Liao: Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, China
Ran Huang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Yi Yang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Yapeng Huang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Kai Zhang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Liang Ma: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Taotao Li: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Taotao Li: Center of Healthy Food Engineering and Technology of Henan, Henan University of Urban Construction, Pingdingshan, Henan, China
Taotao Li: Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, China
Lianzhe Wang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Lianzhe Wang: Center of Healthy Food Engineering and Technology of Henan, Henan University of Urban Construction, Pingdingshan, Henan, China
Lianzhe Wang: Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, China
Huamin Zhang: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Huamin Zhang: Center of Healthy Food Engineering and Technology of Henan, Henan University of Urban Construction, Pingdingshan, Henan, China
Huamin Zhang: Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, China
Bingbing Li: College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, China
Bingbing Li: Center of Healthy Food Engineering and Technology of Henan, Henan University of Urban Construction, Pingdingshan, Henan, China
Bingbing Li: Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, Henan, China

DOI: https://doi.org/10.3389/fmicb.2023.1154811
Journal volume & issue: Vol. 14

Abstract

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Enterobacter cloacae produces insecticidal proteins capable of causing toxicity in pests, but the insecticidal mechanisms of these proteins for insect control remain unclear. To elucidate the mechanisms, the purified insecticidal protein from E. cloacae NK was administered to Galleria mellonella larvae either by intraperitoneal injection or by feeding. The number of hemocytes, apoptosis in immune cells, and polyphenol oxidase (PO) activity of G. mellonella larvae were detected by hemocytometer, Annexin V-FITC/PI, and UV–vis spectrophotometer, respectively. With the extension of the invasion time of NK insecticidal protein, the number of hemocytes in G. mellonella larvae decreased significantly (p < 0.05), whereas the apoptosis rate of hemocytes increased. The activity of PO showed a trend of rising-peak-sharp decline and the melanization reaction was deepened simultaneously. Moreover, the phagocytosis and coating capabilities of hemocytes decreased, and the intraperitoneal injection method was more effective than the feeding method. Taking together, the insecticidal protein of E. cloacae NK inhibits and destroys the cellular immune response of G. mellonella larvae, which suggests an important role in killing the host insect.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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