CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

Yi Fu; Pedro P. Rocha; Vincent M. Luo; Ramya Raviram; Yan Deng; Esteban O. Mazzoni; Jane A. Skok

doi:10.1038/ncomms11707

Nature Communications (May 2016)

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

Yi Fu,
Pedro P. Rocha,
Vincent M. Luo,
Ramya Raviram,
Yan Deng,
Esteban O. Mazzoni,
Jane A. Skok

Affiliations

Yi Fu: Department of Pathology, New York University School of Medicine, 550 First Avenue, Sml 311
Pedro P. Rocha: Department of Pathology, New York University School of Medicine, 550 First Avenue, Sml 311
Vincent M. Luo: Department of Pathology, New York University School of Medicine, 550 First Avenue, Sml 311
Ramya Raviram: Department of Pathology, New York University School of Medicine, 550 First Avenue, Sml 311
Yan Deng: Microscopy Core, New York University School of Medicine
Esteban O. Mazzoni: Department of Biology, New York University
Jane A. Skok: Department of Pathology, New York University School of Medicine, 550 First Avenue, Sml 311

DOI: https://doi.org/10.1038/ncomms11707
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 8

Abstract

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The ability to target Cas9 to specific genomic loci offers the potential for applications beyond genome editing. Here the authors use deactivated Cas9 and chimeric guide RNAs to visualise individual repetitive loci in living cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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