miR-767-5p inhibits the invasion of ovarian cancer cell lines by regulating the expression of TBL1XR1 protein

Abstract

Objective To explore the effect and mechanism of miR-767-5P and transductin β1X linked receptor protein 1 (TBL1XR1) on the progression of ovarian cancer. Methods Database analysis: KEGG (Kyoto Encyclopedia of Genes and Genomes, Kyoto Encyclopedia) enrichment analysis of miR-767-5P using GEO, miRPATH database and RStudio TOP5 microRNAs with differential expression multiples were selected from low and high expression microRNAs in the GEO data set, then ranked from low to high in terms of differential multiples. The target genes for miR-767-5p were predicted by miRDB and other databases. The intersection was selected. The target genes were screened by the Open Target Platform, GEPIA and other databases. GEPIA and THPA databases were used to analyze the expression level of TBL1XR1 in human organs, immunohistochemical analysis and survival curve analysis. Experimental verification: Dual luciferase assay detected the binding target of miR-767-5P and TBL1XR1. Western blot was used to detect TBL1XR1 expression in ovarian cancer cell lines (OC3, SKOV-3, HO-8910) and normal ovarian epithelial cell line(IOSE80). After transferring into SKOV-3 cells with miR-767-5P over-expression plasmid and TBL1XR1 over-expression plasmid, Western blot was used to detect the expression level of TBL1XR1 and Tanswell cell assay was used to detect cell invasion. Results Database analysis: miR-767-5p with the highest differential ratio of low expression was selected as the target microRNA for follow-up research; miR-767-5p was involved in various cancer-related pathways, and miR-767-5p and TBL1XR1 were closely associated with breast cancer and ovarian cancer, etc. TBL1XR1 was highly expressed in ovarian cancer and was associated with poor prognosis. Experimental verification: miR-767-5p and TBL1XR1 could be targetly bound(P＜0.05); Compared with IOSE80, TBL1XR1 was significantly over-expressed in OC3, SKOV-3 and HO-8910 cells. After the transfection of miR-767-5p over-expressed plasmid, the expression level of TBL1XR1 was decreased and the aggressiveness of ovarian cancer cells was reduced(P＜0.05), while simultaneously transfection of TBL1XR1 over-expressed plasmid could reduce this effect to some extent. Conclusions miR-767-5p regulates TBL1XR1 expression and inhibits the invasion of human ovarian cancer cell lines.

Keywords

• ovarian cancer|invasion|mir-767-5p|transductin beta-like 1 x-linked receptor(tbl1xr1)