A suitable enzymatic method for starch quantification in different organic matrices

Abstract

Starch can represent 70–80% of the cereals grains (on a dry matter basis) used for livestock feeding. Several methods have been developed to estimate the feed starch contents of energy feed sources. However, the efficiency of these methods to evaluate the starch content in other feed sources, as well as other types of samples used to evaluate starch availability in the gastrointestinal tract, such as digesta and faeces, remains unclear. Furthermore, most of the currently used starch analysis methods have not been effectively evaluated, being only applied to samples of sporadic experiments, without a wide-ranging validation of the procedures and results. Here, we propose a modification of a method for analysing the starch content in different organic matrices normally evaluated in ruminant nutrition studies. The evaluated organic matrices were: soybean meal, soybean hull, Tifton 85 Bermuda grass hay, abomasal digesta, and faeces. • The modified method is more feasible than the original procedures. • The modified method estimates the starch contents in different organic matrices with accuracy and precision. Method name: An enzymatic method for starch analysis, Keywords: Feed analysis, Glucose, Ruminant faeces, Ruminant digesta