Development and Evaluation of Novel Alginate-based Photocrosslinkable Tissue Adhesive: An In-vitro Study

Abstract

Introduction: In clinical practice, traditional methods like sutures and staples are employed to halt bleeding and expedite wound healing. However, these techniques, which involve piercing tissue, come with drawbacks including the risk of inflammation, infections, and the formation of scars. Surgical sealants and tissue adhesives offer a way to mitigate some of these disadvantages associated with conventional sutures and staples. Tissue adhesives have emerged as a newer alternative for non invasive wound closure. Aim: To develop and analyse the properties of alginate-based photocrosslinkable tissue adhesive. Materials and Methods: This in-vitro analysis was done at the Department of Periodontology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India from April 2022 to May 2022. The tissue adhesive was prepared by mixing Alginate, N-Hydroxysuccinamide (NHS) and 1-Ethyl, 3- Dimethylaminopropyl Carbodiimide (EDC) and methacrylate which was used as photo initiator. The preparation was followed by characterisation of the material, strength test and evaluation of biocompatibility. Results: Surface characteristics test showed homogeneous slices in Scanning Electron Microscope (SEM) analysis. Mechanical test compressive strain was found to be 3% for both the specimens whereas tensile strain was 211.40% and 269.50% for specimen 1 and 2, respectively. Biocompatibility test of the newly developed adhesive showed that adhesive caused the L929 cells to multiply less than the fibrin glue. Conclusion: All these results suggested that the developed material is a promising soft tissue adhesive for various applications in dentistry.

Keywords

• biomaterial
• innovative tissue adhesive
• natural polymers
• wound closure