Scopoletin Induced Metabolomic Profile Disturbances in Zebrafish Embryos

Weixuan Yao; Jingpei Chen; Zhanyu Lin; Nani Wang; Anli Wang; Binjie Wang; Yuanzhao Wu; Zhongshi Xu; Jiye Wang

doi:10.3390/metabo12100934

Metabolites (Oct 2022)

Scopoletin Induced Metabolomic Profile Disturbances in Zebrafish Embryos

Weixuan Yao,
Jingpei Chen,
Zhanyu Lin,
Nani Wang,
Anli Wang,
Binjie Wang,
Yuanzhao Wu,
Zhongshi Xu,
Jiye Wang

Affiliations

Weixuan Yao: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Jingpei Chen: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Zhanyu Lin: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Nani Wang: Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310012, China
Anli Wang: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Binjie Wang: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Yuanzhao Wu: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Zhongshi Xu: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Jiye Wang: Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, The Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China

DOI: https://doi.org/10.3390/metabo12100934
Journal volume & issue: Vol. 12, no. 10
p. 934

Abstract

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Scopoletin, a typical example of a coumarin compound, exists in several Artemisia species and other plant genera. However, the systemic metabolic effects induced by scopoletin remain unclear. In the present study, we evaluated the metabolic profiles in scopoletin-exposed zebrafish embryos using UHPLC-Q-Obitrap-HRMS combined with multivariate analysis. Compared with the control group, 33 metabolites in scopoletin group were significantly upregulated, while 27 metabolites were significantly downregulated. Importantly, scopoletin exposure affected metabolites mainly involved in phosphonate and phosphinate metabolism, vitamin B6 metabolism, histidine metabolism, sphingolipid metabolism, and folate biosynthesis. These results suggested that scopoletin exposure to zebrafish embryos exhibited marked metabolic disturbance. This study provides a perspective of metabolic impacts and the underlying mechanism associated with scopoletin exposure.

Published in Metabolites

ISSN: 2218-1989 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/metabolites

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