Cellular Physiology and Biochemistry (Jun 2018)

Generation and Role of Oscillatory Contractions in Mouse Airway Smooth Muscle

  • Hao Xu,
  • Ping Zhao,
  • Wen-Jing Zhang,
  • Jun-Ying Qiu,
  • Li Tan,
  • Xiao-Cao Liu,
  • Qian Wang,
  • Xi Luo,
  • Yu-Shan She,
  • Dun-An Zang,
  • Bei-Bei Liu,
  • Lei Cao,
  • Xiao-Xue Zhao,
  • Yuan-Yuan Chen,
  • Meng-Yue Li,
  • Jinhua Shen,
  • Yong-Bo Peng,
  • Lu Xue,
  • Meng-Fei Yu,
  • Weiwei Chen,
  • Li-Qun Ma,
  • Gangjian Qin,
  • Qing-Hua Liu

DOI
https://doi.org/10.1159/000490873
Journal volume & issue
Vol. 47, no. 4
pp. 1546 – 1555

Abstract

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Background/Aims: Tetraethylammonium chloride (TEA) induces oscillatory contractions in mouse airway smooth muscle (ASM); however, the generation and maintenance of oscillatory contractions and their role in ASM are unclear. Methods: In this study, oscillations of ASM contraction and intracellular Ca2+ were measured using force measuring and Ca2+ imaging technique, respectively. TEA, nifedipine, niflumic acid, acetylcholine chloride, lithium chloride, KB-R7943, ouabain, 2-Aminoethoxydiphenyl borate, thapsigargin, tetrodotoxin, and ryanodine were used to assess the mechanism of oscillatory contractions. Results: TEA induced depolarization, resulting in activation of L-type voltage-dependent Ca2+ channels (LVDCCs) and voltage-dependent Na+ (VNa) channels. The former mediated Ca2+ influx to trigger a contraction and the latter mediated Na+ entry to enhance the contraction via activating LVDCCs. Meanwhile, increased Ca2+-activated Cl- channels, inducing depolarization that resulted in contraction through LVDCCs. In addition, the contraction was enhanced by intracellular Ca2+ release from Ca2+ stores mediated by inositol (1,4,5)-trisphosphate receptors (IP3Rs). These pathways together produce the contractile phase of the oscillatory contractions. Furthermore, the increased Ca2+ activated the Na+-Ca2+ exchanger (NCX), which transferred Ca2+ out of and Na+ into the cells. The former induced relaxation and the latter activated Na+/K+-ATPase that induced hypopolarization to inactivate LVDCCs causing further relaxation. This can also explain the relaxant phase of the oscillatory contractions. Moreover, the depolarization induced by VNa channels and NCX might be greater than the hypopolarization caused by Na+/K+-ATPase alone, inducing LVDCC activation and resulting in further contraction. Conclusions: These data indicate that the TEA-induced oscillatory contractions were cooperatively produced by LVDCCs, VNa channels, Ca2+-activated Cl- channels, NCX, Na+/K+ ATPase, IP3Rs-mediated Ca2+ release, and extracellular Ca2+.

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