PLoS Pathogens (Jan 2012)

Necrotrophism is a quorum-sensing-regulated lifestyle in Bacillus thuringiensis.

  • Thomas Dubois,
  • Karoline Faegri,
  • Stéphane Perchat,
  • Christelle Lemy,
  • Christophe Buisson,
  • Christina Nielsen-LeRoux,
  • Michel Gohar,
  • Philippe Jacques,
  • Nalini Ramarao,
  • Anne-Brit Kolstø,
  • Didier Lereclus

DOI
https://doi.org/10.1371/journal.ppat.1002629
Journal volume & issue
Vol. 8, no. 4
p. e1002629

Abstract

Read online

How pathogenic bacteria infect and kill their host is currently widely investigated. In comparison, the fate of pathogens after the death of their host receives less attention. We studied Bacillus thuringiensis (Bt) infection of an insect host, and show that NprR, a quorum sensor, is active after death of the insect and allows Bt to survive in the cadavers as vegetative cells. Transcriptomic analysis revealed that NprR regulates at least 41 genes, including many encoding degradative enzymes or proteins involved in the synthesis of a nonribosomal peptide named kurstakin. These degradative enzymes are essential in vitro to degrade several substrates and are specifically expressed after host death suggesting that Bt has an active necrotrophic lifestyle in the cadaver. We show that kurstakin is essential for Bt survival during necrotrophic development. It is required for swarming mobility and biofilm formation, presumably through a pore forming activity. A nprR deficient mutant does not develop necrotrophically and does not sporulate efficiently in the cadaver. We report that necrotrophism is a highly regulated mechanism essential for the Bt infectious cycle, contributing to spore spreading.