Veterinary World (May 2020)

Molecular characterization and phylogenetic analysis of fowl adenovirus serotype-4 from Guangdong Province, China

  • Fu Yuming,
  • Yuan Sheng,
  • Deng Wenyu,
  • Chi Shihong,
  • Li Wenfeng,
  • Huang Wenjing,
  • Li Xiaowen,
  • Saeed El-Ashram,
  • Kun Mei,
  • Guo Jinyue,
  • Zhang Xuelian,
  • Li Zhili,
  • Huang Shujian

DOI
https://doi.org/10.14202/vetworld.2020.981-986
Journal volume & issue
Vol. 13, no. 5
pp. 981 – 986

Abstract

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Aim: Our aim in this study was to isolate potentially novel strains of fowl adenovirus serotype-4 (FAdV-4) that is currently circulating in broiler chicken flocks in Guangdong Province, China, and to compare nucleotide and amino acid (AA) sequences of their respective hexon genes. Materials and Methods: The experiment was carried out on poultry farms experiencing outbreaks of FAdV-4-associated hydropericardium syndrome (HPS). Tissue samples from the hearts and livers of deceased chickens were screened for FAdV-4 infection using hexon gene-specific polymerase chain reaction (PCR). Results: New virus isolates were used to infect 7-day-old chicks, which went onto reproduce typical HPS signs. The hypervariable region of the FAdV-4 hexon gene was PCR-amplified and sequenced. The hexon nucleotide and deduced AA sequence identities were 99.8-99.9% and 99.5-99.8%, respectively, among the four novel isolates. In addition, the new isolates were 97-100% and 96.4-99.9% identical to the nucleotide and deduced AA sequences, respectively, of FAdV-4 hexon genes available in the National Center for Biotechnology Information GenBank database. Phylogenetic analyses, based on the hexon gene sequence, revealed that the new isolates, clustered with FAdV-C; the FAdV-A, FAdV-B, FAdV-D, and FAdV-E viruses, were more distantly related. Conclusion: New FAdV-4 isolates from Guangdong Province are similar to those identified in other regions of the world. This information provides critical insight into HPS epidemiology and provides a perspective for monitoring outbreaks and developing strategies for disease prevention.

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